December 2006
Volume 6, Issue 13
Free
OSA Fall Vision Meeting Abstract  |   December 2006
Depth-resolved optical probing of retinal physiology with functional ultrahigh resolution optical coherence tomography
Author Affiliations
  • Kostadinka Bizheva
    Department of Physics and Astronomy, University of Waterloo, Waterloo, Canada, and Center of Biomedical Engineering, Medical University of Vienna, Vienna, Austria
  • R. Pflug
    Physiology, Medical University of Vienna
  • B. Hermann
    Center of Biomedical Engineering, Medical University of Vienna
  • B. Povazay
    Center of Biomedical Engineering, Medical University of Vienna
  • H. Sattmann
    Center of Biomedical Engineering, Medical University of Vienna
  • P. Qui
    Center of Biomedical Engineering, Medical University of Vienna
  • E. Anger
    Physiology, Medical University of Vienna
  • H. Reitsamer
    Physiology, Medical University of Vienna
  • S. Popov
    Physics, Imperial College
  • J. R. Taylor
    Physics, Imperial College
  • A. Unterhuber
    Center of Biomedical Engineering, Medical University of Vienna
  • P. Ahnelt
    Physiology, Medical University of Vienna
  • W. Drexler
    Center of Biomedical Engineering, Medical University of Vienna
Journal of Vision December 2006, Vol.6, 40. doi:10.1167/6.13.40
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      Kostadinka Bizheva, R. Pflug, B. Hermann, B. Povazay, H. Sattmann, P. Qui, E. Anger, H. Reitsamer, S. Popov, J. R. Taylor, A. Unterhuber, P. Ahnelt, W. Drexler; Depth-resolved optical probing of retinal physiology with functional ultrahigh resolution optical coherence tomography. Journal of Vision 2006;6(13):40. doi: 10.1167/6.13.40.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Non-contact, depth-resolved, optical probing of retinal response to visual stimulation with a <10µm spatial resolution, achieved by using functional ultrahigh-resolution optical coherence tomography (fUHROCT), is demonstrated in isolated rabbit retinas. The method takes advantage of the fact that physiological changes in dark-adapted retinas caused by light stimulation can result in local variation of the tissue reflectivity. fUHROCT scans were acquired from isolated retinas synchronously with electrical recordings before, during, and after light stimulation. Pronounced stimulus related changes in the retinal reflectivity profile were observed in the inner / outer segments of the photoreceptor layer and the plexiform layers. Control experiments (e.g., dark adaptation vs. light stimulation), pharmacological inhibition of photoreceptor function, and synaptic transmission to the inner retina confirmed that the origin of the observed optical changes is the altered physiological state of the retina evoked by the light stimulus. The conducted experiments demonstrated that fUHROCT allows for simultaneous, noninvasive probing of both retinal morphology and function, which could significantly improve the early diagnosis of various ophthalmic pathologies and could lead to better understanding of pathogenesis.

In the past year, advances in UHROCT technology have allowed conducting in-vivo fUHROCT measurements in animal models and human patients, thus bringing the fUHROCT technique closer to clinical trials and commercial development.

Bizheva, K. Pflug, R. Hermann, B. Povazay, B.P. Sattmann, H. Qui, P. Anger, E. Reitsamer, H. Popov, S. Taylor, J. R. Unterhuber, A. Ahnelt, P. Drexler, W. (2006). Depth-resolved optical probing of retinal physiology with functional ultrahigh resolution optical coherence tomography [Abstract]. Journal of Vision, 6(13):40, 40a, http://journalofvision.org/6/13/40/, doi:10.1167/6.13.40. [CrossRef]
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