December 2007
Volume 7, Issue 15
Free
OSA Fall Vision Meeting Abstract  |   December 2007
Interkinetic nuclear migration, cell polarity, and retinal neurogenesis
Author Affiliations
  • Brian Link
    Medical College of Wisconsin
  • Shuang Cui
    Medical College of Wisconsin
  • Lisa Baye
    Medical College of Wisconsin
Journal of Vision December 2007, Vol.7, 30. doi:10.1167/7.15.30
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to Subscribers Only
      Sign In or Create an Account ×
    • Get Citation

      Brian Link, Shuang Cui, Lisa Baye; Interkinetic nuclear migration, cell polarity, and retinal neurogenesis. Journal of Vision 2007;7(15):30. doi: 10.1167/7.15.30.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

During retinal development, neuroepithelial progenitor cells divide in either a symmetric proliferative mode, where both daughter cells remain mitotic, or in a neurogenic mode, where at least one daughter cell exits the cell cycle and differentiates as a neuron. While the cellular mechanisms of neurogenesis remain unknown, heterogeneity in cell behaviors has been postulated to influence this cell fate. I will present experiments from our lab analyzing interkinetic nuclear migration, the apical-basal movement of nuclei in phase with the cell cycle, and describe relationships of this cell behavior to neurogenesis. Using time-lapse imaging in zebrafish we find that various parameters of interkinetic nuclear migration are significantly heterogeneous among retinal neuroepithelial cells. Of significance, neurogenic progenitors have greater basal nuclei migrations during the last cell cycle preceding a terminal mitosis. In addition, we show that aPKC-mediated cell polarity is essential for the relationship between nuclear position and neurogenesis. Loss of aPKC also resulted in increased proliferative cell divisions and reduced retinal neurogenesis. Cumulatively, our data supports a novel model for neurogenesis where interkinetic nuclear migration differentially positions nuclei in neuroepithelial cells and therefore influences selection of progenitors for cell cycle exit based on apical-basal polarized signals.

Link, B. Cui, S. Baye, L. (2007). Interkinetic nuclear migration, cell polarity, and retinal neurogenesis [Abstract]. Journal of Vision, 7(15):30, 30a, http://journalofvision.org/7/15/30/, doi:10.1167/7.15.30. [CrossRef]
Footnotes
 NIH/NEI R01EY014167
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×