December 2007
Volume 7, Issue 15
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OSA Fall Vision Meeting Abstract  |   December 2007
In Vivo Labeling of Mammalian Cone Photoreceptors by Intravitreal Injection of Fluorescently Tagged Peanut Agglutinin - A Potential Tool for Assessing Photoreceptor Degeneration in Humans
Author Affiliations
  • Vidhyasankar Krishnamoorthy
    National Brain Research Center, Manesar (Haryana) 122050, In
  • Cherukuri
    National Brain Research Center, Manesar (Haryana) 122050, India
  • Sonia Baloni
    National Brain Research Center, Manesar (Haryana) 122050
  • Narender Dhingra
    India National Brain Research Center, Manesar (Haryana) 122050, India
Journal of Vision December 2007, Vol.7, 75. doi:10.1167/7.15.75
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      Vidhyasankar Krishnamoorthy, Cherukuri, Sonia Baloni, Narender Dhingra; In Vivo Labeling of Mammalian Cone Photoreceptors by Intravitreal Injection of Fluorescently Tagged Peanut Agglutinin - A Potential Tool for Assessing Photoreceptor Degeneration in Humans. Journal of Vision 2007;7(15):75. doi: 10.1167/7.15.75.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Retinal degenerative diseases, such as Retinitis Pigmentosa and Age-Related Macular Degeneration are characterized by selective degeneration of photoreceptors. Routine clinical methods neither allow confirmed diagnosis nor an assessment of extent and severity of the degeneration. We asked if Peanut Agglutinin (PNA) could selectively label mammalian retinal cones in vivo, when administered intravitreally. We injected FITC-conjugated PNA intravitreally into anesthetized mouse, guinea pig or monkey, and found it to stain cones, selectively and reversibly. To examine if PNA affected other retinal neurons, we immunostained them for several retinal cell markers, and found their expression indistinguishable from normal. TUNEL assay did not show any PNA-induced apoptosis. Visual behavior of animals in a Visual Cliff test 10 hours after the PNA injection showed no impairment. Finally, PNA-stained cones in an animal model of photoreceptor degeneration showed a pattern that reflected progressive degeneration. Together, these findings demonstrate that intravitreally injected PNA can selectively bind to mammalian cones in vivo without causing, at least any overt adverse effects. We propose this as a potential tool to make a confirmed diagnosis, and to assess the extent and severity of photoreceptor degeneration in some of the retinal degenerative diseases in humans.

Krishnamoorthy, V. Pitchaiah, C. Baloni, S. Dhingra, N. (2007). In Vivo Labeling of Mammalian Cone Photoreceptors by Intravitreal Injection of Fluorescently Tagged Peanut Agglutinin - A Potential Tool for Assessing Photoreceptor Degeneration in Humans [Abstract]. Journal of Vision, 7(15):75, 75a, http://journalofvision.org/7/15/75/, doi:10.1167/7.15.75. [CrossRef]
Footnotes
 This work was supported by grants from NBRC and DBT (BT/PR6615/MED/14/857/2005)
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