December 2007
Volume 7, Issue 15
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OSA Fall Vision Meeting Abstract  |   December 2007
Functional Magnetic Resonance Imaging of Neural Activity in Rat CNS in Response to Chromatic Stimuli
Author Affiliations
  • Matthew Mauck
    Departments of Ophthalmology and Cell Biology, Neurobiology
  • James Kuchenbecker
    Department of Ophthalmology, Medical College of Wisconsin
  • Christopher Pawela
    Department of Biophysics, Medical College of Wisconsin
  • James Hyde
    Department of Biophysics, Medical College of Wisconsin
  • Anthony Hudetz
    Department of Anesthesiology, Medical college of Wisconsin
  • Maureen Neitz
    Department of Ophthalmology, Medical College of Wisconsin
  • Jay Neitz
    Department of Ophthalmology, Medical College of Wisconsin
Journal of Vision December 2007, Vol.7, 82. doi:10.1167/7.15.82
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      Matthew Mauck, James Kuchenbecker, Christopher Pawela, James Hyde, Anthony Hudetz, Maureen Neitz, Jay Neitz; Functional Magnetic Resonance Imaging of Neural Activity in Rat CNS in Response to Chromatic Stimuli. Journal of Vision 2007;7(15):82. doi: 10.1167/7.15.82.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

In rodents, dichromatic color vision is based on comparisons of M-cones and UV-cones. However, little is known about the location and distribution of cortical regions carrying color information in the rodent. Therefore, we used functional magnetic resonance imaging (fMRI) to measure the blood-oxygenation level dependent (BOLD) signal generated by a heterochromatically modulated stimulus. Two stimulus conditions were designed to favor either luminance or chromatic pathways individually. M-cone quantal catches of an ultraviolet light (DW= 465 nm) were adjusted to match those of a green light (DW= 545 nm). The lights were modulated in counter-phase at 1 Hz to isolate chromatic signals produced by S-cones. To acquire a response to luminance increments and decrements, 545 nm light was modulated at 1 Hz without the 465 nm light. BOLD responses were measured for each stimulus condition. Diffuse activation in V1 with lesser activation in the superior colliculus and the dorsal lateral geniculate (DLG) nucleus was observed in response to the chromatic condition. The luminance condition produced a more robust activation of the superior colliculus and DLG. This approach will allow us to evaluate the functional consequences of newly introduced opsin molecules targeted to either M- or S- photoreceptors in rodents.

Mauck, M. Kuchenbecker, J. Pawela, C. Hyde, J. Hudetz, A. Neitz, M. Neitz, J. (2007). Functional Magnetic Resonance Imaging of Neural Activity in Rat CNS in Response to Chromatic Stimuli [Abstract]. Journal of Vision, 7(15):82, 82a, http://journalofvision.org/7/15/82/, doi:10.1167/7.15.82. [CrossRef]
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