December 2013
Volume 13, Issue 15
Free
OSA Fall Vision Meeting Abstract  |   October 2013
Temporal properties of photopigment contributions to the pupillary light reflex
Author Affiliations
  • Manuel Spitschan
    Department of Psychology, University of Pennsylvania, Philadelphia, Pennsylvania, USA
  • Sandeep Jain
    Department of Neurology, University of Pennsylvania, Philadelphia, Pennsylvania, USA
  • David H. Brainard
    Department of Psychology, University of Pennsylvania, Philadelphia, Pennsylvania, USA
  • Geoffrey K. Aguirre
    Department of Neurology, University of Pennsylvania, Philadelphia, Pennsylvania, USA
Journal of Vision October 2013, Vol.13, P4. doi:10.1167/13.15.39
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      Manuel Spitschan, Sandeep Jain, David H. Brainard, Geoffrey K. Aguirre; Temporal properties of photopigment contributions to the pupillary light reflex. Journal of Vision 2013;13(15):P4. doi: 10.1167/13.15.39.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

The pupillary light reflex (PLR) regulates the amount of light entering the eye. It is driven by the activation of rods, cones, and intrinsically photosensitive ganglion cells (ipRGCs), with the latter expressing the melanopsin photopigment (λmax = 480 nm). The degree to which these different receptors contribute to the PLR and their relative temporal filtering properties are addressed in this study.

The temporal roll-off in pupillary response amplitude was measured using sinusoidally modulated stimuli at approximately logarithmically spaced temporal frequencies (0.01, 0.05, 0.1, 0.5, 1 and 2 Hz) displayed in the peripheral visual field (27.5° field size, central 5° blackened). The spectral composition of the stimuli was controlled with a device allowing for the construction of arbitrary spectral power distributions (OneLight Digital Light Engine). Modulations were directed at L and M cones (L+M) and melanopsin while silencing the other photopigment classes with the method of silent substitution. Two observers viewed the stimulus with their left eye pharmacologically dilated and with a 4.7 mm artificial pupil. The consensual pupillary response of their right eye was measured with an IR eye tracker (Cambridge Research Systems).

The amplitude of pupil response was measured for each modulation frequency and photopigment-directed stimulus. The resulting temporal transfer functions demonstrate different roll-off properties for L+M cone and melanopsin-directed stimulation. Specifically, L+M driven responses are temporally bandpass with maximal pupil modulation in response to 0.1 Hz stimulation, while melanopsin responses are temporally low-pass.

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