The method we used for measuring interocular suppression using random-dot kinematograms has been described in detail elsewhere.
13 Briefly, stimuli were displayed using a video goggle apparatus (Z800 3D Visor; eMagin Corp., Washington, DC) driven by a laptop computer (MacBook Pro; Apple Computer, Cupertino, CA, running MatLab; The MathWorks, Natick, MA) and the Psychophysics Toolbox, version 3.
12,13,16 This apparatus allowed for separate images to be presented to each eye and for the images in each eye to be aligned by the participants, using routines within the stimulus presentation software. Stimuli were random-dot kinematograms, which consisted of a population of signal dots, all moving in a common direction, and a population of noise dots, that moved randomly. Dots were bright against a mean luminance background (35 cd/m
2). The luminance modulation (Michelson contrast) and hence the visibility of the dots could be varied by increasing the luminance of the dots, with respect to the background, according to the following equation:
where
L dots and
L background are the dot and background luminance, respectively. Signal dots were presented to one eye, and noise dots were presented to the other eye. The task was to indicate the motion direction of the signal dots. A staircase procedure controlled the relative proportion of signal-to-noise dots in the stimulus to allow for the measurement of a motion coherence threshold (the number of signal dots required for 71% correct performance; see Black et al.
13 for further details [their method 1] and illustrative figures of this technique). To measure suppression, the contrast of the dots presented to the amblyopic eye was fixed at 100% whereas the contrast of the dots presented to the fellow fixing eye was varied across five contrast levels (100%, 80%, 50%, 25%, and 12.5% contrast, equivalent to dot luminances of 70, 63, 52.5, 43.8, and 39.4 cd/m
2, respectively), using the method of constant stimuli. Within a single measurement session, 10 randomly interleaved staircases were presented, five for each contrast level with the signal dots shown to the amblyopic eye and five for the signal dot presentation to the fellow fixing eye. Two measurement sessions were conducted per patient separated by a 30-minute break. The fellow fixing eye contrast at which the motion coherence thresholds were the same irrespective of which eye saw the signal and which saw the noise was calculated by fitting linear functions to the average threshold data for each eye as a function of fellow fixing eye contrast and calculating the intersection of these fits.
13 We refer to this dichoptic contrast offset as the “balance point,” as it represents the point at which suppression has been overcome and information is being combined between the two eyes in a normal fashion.
12,13 Therefore, the balance point contrast can be considered as a parametric measurement of suppression.
12 For the control group the nondominant eye, as defined by the hole-in-the-card test, was designated as the amblyopic eye for these measurements. The alignment of central nonius lines (one to each eye) was used to ensure accurate alignment of the stimulus fields seen by the right and left eyes. Subjects were asked to attend to the central part of the stimulus field. The fact that for this stimulus corresponding points are not stimulated (i.e., the signal and noise dots do not overlap in space) allows fusion to occur on a more global level, and we believe it is this that makes its use as a treatment so effective. We view the point-wise suppression as more of V1 function and the global suppression more of extrastriate function.