December 2017
Volume 17, Issue 15
Open Access
OSA Fall Vision Meeting Abstract  |   December 2017
Photoreceptor pathways differentially contribute to refractive eye growth in mice
Author Affiliations
  • Machelle Pardue
    Wallace H. Coulter, Department of Biomedical Engineering, Georgia Institute of Technology
    Rehab R&D Center of Excellence, Atlanta VA Medical Center
  • Ranjay Chakraborty
    Rehab R&D Center of Excellence, Atlanta VA Medical Center
    Ophthalmology, Emory University School of Medicine
  • Erica Landis
    Rehab R&D Center of Excellence, Atlanta VA Medical Center
    Ophthalmology, Emory University School of Medicine
  • Hanna Park
    Rehab R&D Center of Excellence, Atlanta VA Medical Center
    Ophthalmology, Emory University School of Medicine
  • Richard A. Stone
    Ophthalmology, University of Pennsylvania
  • P. Michael Iuvone
    Ophthalmology, Emory University School of Medicine
    Pharmacology, Emory University School of Medicine
Journal of Vision December 2017, Vol.17, 34. doi:10.1167/17.15.34a
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      Machelle Pardue, Ranjay Chakraborty, Erica Landis, Hanna Park, Richard A. Stone, P. Michael Iuvone; Photoreceptor pathways differentially contribute to refractive eye growth in mice. Journal of Vision 2017;17(15):34. doi: 10.1167/17.15.34a.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

The specific retinal pathways that detect visual input and initiate the signaling for refractive development are unknown. We investigated the contributions of the three photoreceptor pathways in refractive development by testing photoreceptor specific mutant models and by selectively stimulating photoreceptor pathways using ambient lighting.

Refractions, corneal curvatures, and ocular parameters were measured in mice with non-functional rods (Gnat1−/−), cones (Gnat2−/−), or intrinsically photosensitive retinal ganglion cells (ipRGCs) (Opn4−/−) and compared to wild-type (WT) age-matched controls. Mice experienced normal laboratory conditions (NLC) or monocular form deprivation (FD) from P28 to P84. Separate cohorts of WT mice were housed under 0.005, 50 or 15,000 lux ambient lighting starting at P28. Half of these mice underwent monocular lens defocus for one week.

Gnat1−/− mice had abnormal refractive development under NLC and no response to FD compared to WT mice (p<0.001). In contrast, Gnat2−/− mice had responses similar to WT with NLC (p=n.s.) and FD (p=0.04). Opn4−/− mice had abnormal refractive development with NLC and significantly greater FD-induced myopic shifts than WT (p<0.001). The different ambient conditions did not alter refractive development with NLC. However, 0.005 lux and 15,000 lux conditions reduced the lens-induced myopic shifts compared to 50 lux lighting (p<0.05).

Our findings suggest that photoreceptor pathways differentially influence refractive development in mice. Disruption of rod and ipRGC pathways, but not cone pathways, significantly alters refractive development under NLC and FD conditions, though in different directions. Rod pathway signaling is important for refractive development and may even provide protection from induced myopia.

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