December 2019
Volume 19, Issue 15
Open Access
OSA Fall Vision Meeting Abstract  |   December 2019
Thyroid hormone signaling specifies cone subtypes in human retinal organoids
Author Affiliations
  • Robert J. Johnston
    Department of Biology, Johns Hopkins University, Baltimore, MD
Journal of Vision December 2019, Vol.19, 15. doi:https://doi.org/10.1167/19.15.15
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      Robert J. Johnston; Thyroid hormone signaling specifies cone subtypes in human retinal organoids. Journal of Vision 2019;19(15):15. doi: https://doi.org/10.1167/19.15.15.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

The mechanisms underlying specification of neuronal subtypes within the human nervous system are largely unknown. The blue (S), green (M), and red (L) cones of the retina enable high-acuity daytime and color vision. To determine the mechanism that controls S versus L/M fates, we studied the differentiation of human retinal organoids. Organoids and retinas have similar distributions, expression profiles, and morphologies of cone subtypes. S cones are specified first, followed by L/M cones, and thyroid hormone signaling controls this temporal switch. Dynamic expression of thyroid hormone-degrading and -activating proteins within the retina ensures low signaling early to specify S cones and high signaling late to produce L/M cones. This work establishes organoids as a model for determining mechanisms of human development with promising utility for therapeutics and vision repair.

Science2018Oct123626411
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