September 2021
Volume 21, Issue 9
Open Access
Vision Sciences Society Annual Meeting Abstract  |   September 2021
Imaging of calcium indicators in retinal ganglion cells for understanding foveal function
Author Affiliations
  • Juliette E. McGregor
    Centre for Visual Science, University of Rochester
Journal of Vision September 2021, Vol.21, 1808. doi:https://doi.org/10.1167/jov.21.9.1808
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Juliette E. McGregor; Imaging of calcium indicators in retinal ganglion cells for understanding foveal function. Journal of Vision 2021;21(9):1808. https://doi.org/10.1167/jov.21.9.1808.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

The fovea mediates much of our conscious visual perception but is a delicate retinal structure that is difficult to investigate physiologically using traditional approaches. By expressing the calcium indicator protein GCaMP6s in retinal ganglion cells (RGCs) of the living primate we can optically read out foveal RGC activity in response to visual stimuli presented to the intact eye. Pairing this with adaptive optics ophthalmoscopy it is possible to both present highly stabilized visual stimuli to the fovea and read out retinal activity on a cellular scale in the living animal. This approach has allowed us to map the functional architecture of the fovea at the retinal level and to classify RGCs in vivo based on their responses to chromatic stimuli. Recently we have used this platform as a pre-clinical testbed to demonstrate successful restoration of foveal RGC responses following optogenetic therapy.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×