Depth constancy is said to occur when perceived depth magnitude is constant across viewing distance. Note that the estimates need not be veridical, there may be a constant offset at all distances. To determine if observers attained depth constancy, we compared the intercepts and slopes of each function between the near and far viewing distances for each group of observers. For observers that estimated the depth of physical half-cylinders first, the slopes of depth estimates for virtual stimuli in the stereoscope, b = −0.15, t(62) = −2.33, p = 0.02, r = 0.28, and HMD, b = −0.17, t(62) = −2.05, p = 0.04, r = 0.25, were significantly different at the near and far viewing distances. However, for physical stimuli the intercept, b = −0.38, t(7) = −0.67, p = 0.52, r = 0.25, and slope, b = −0.01, t(46) = −0.12, p = 0.91, r = 0.02, of depth estimates were consistent at the two viewing distances. For observers that viewed virtual half-cylinders first, the slopes of perceived depth estimates for the stereoscope, b = −0.18, t(62) = −3.44, p = 0.001, r = 0.40, HMD, b = −0.13, t(62) = −3.30, p = 0.002, r = 0.39, and physical viewing conditions, b = −0.20, t(46) = −2.08, p = 0.04, r = 0.26, were significantly more shallow in the far viewing distance than to the near viewing distance. In sum, stereoscopic depth constancy was only seen in the physical-first conditions when physical stimuli were being tested. Despite the similarity of the stimuli, constancy was never attained in any of the virtual test conditions.